N-methyl-D-aspartate 수용기의 다양한 조절이 일차 배양된 정상사람구강각화세포의 증식에 미치는 영향 DNA 합성율 평가
The effect of differential modulation of N-methyl-D-aspartate receptor on the proliferation of primary cultured normal human oral keratinocytes: DNA synthesis rate analysis
김인수, 백기석, 장미숙, 이원, 이승표,
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김인수 ( Kim In-Soo ) - 가톨릭대학교 의정부성모병원 치과 구강악안면외과
백기석 ( Paik Ki-Suk ) - 서울대학교 치과대학 구강해부학교실
장미숙 ( Chang Mi-Sook ) - 서울대학교 치과대학 구강해부학교실
이원 ( Lee Won ) - 가톨릭대학교 의정부성모병원 치과 구강악안면외과
이승표 ( Lee Seung-Pyo ) - 서울대학교 치과대학 구강해부학교실
KMID : 0355620070330020124
Abstract
In the present study, I investigated the effects of N-methyl-D-aspartate (NMDA), arachidonic acid (AA), and Nitric Oxide Synthase Inhibitor (NOSI), alone or in combination, on the proliferation of cultured primary normal human oral keratinocytes (NHOK). The purpose of this study was therefore the preliminary study for the examination of the interaction between these agents and NHOK in order to elucidate the mechanisms by which epithelial growth and regeneration are regulated. NHOK were obtained from gingival tissue of 20 individuals aged 20 to 29, and third passage (P3) cells were used for this study. The DNA synthesis was measured by the BrdU assay. Addition of low concentration of AA (1 ?M) and high concentration of AA with NMDA group (NMDA+AA 10 ?M) made DNA synthesis rate increase significantly at the early stage. Adding NNA (10 ?M) affected DNA synthesis rate to increase significantly in 4 hours. At the early stage, DNA synthesis was significantly active in the NOS-I with NMDA groups than in the control and the NMDA-only group, while it didn’t become statistically meaningful in 24 hours. AA 1 ?M and NNA 10 ?M may induce the proliferation of the NHOK independently and NOS-I may induce the proliferation of the NHOK with NMDA. These reactions might be related to the NMDA receptor in the cell and the change of the intracellular calcium ion concentration.
키워드
N-methyl-D-aspartate;proliferation;Normal Human Oral Keratinocyte;Arachidonic acid;Nitric Oxide Synthase Inhibitor
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